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Gate Questions for Biochemistry likely to be asked for Gate 2014 is given here. Sample Questions given here will be helpful for all writing life sciences XL paper in Gate 2014. Model Question Papers with Answers for Biochemistry have been prepared affter evaluating previous years questions in Gate.


The actual free energy change of a given biochemical reaction carried out under standard 
conditions with 1 M initial concentration of each of the reactants and products will be 
(A) equal to zero
(B) equal to standard free energy change for the reaction
(C) less than the standard free energy change for the reaction
(D) greater than the standard free energy change for the reaction 

Which one of the following closely defines ‘Molten Globule’ state of a protein?
(A) State with high degree of secondary structure and loss of tertiary structure
(B) State with complete loss of secondary structure
(C) Completely unfolded state
(D) Loss of quaternary structure

Which one of the following amino-acids has highest fluorescence quantum yield (F) in aqueous 
(A) Tyrosine (B) Tryptophan (C) Phenylalanine (D) Histidine

The pair of amino-acids which does NOT undergo post-translational modification is
(A) Asn-His (B) Tyr-Ser (C) Asn-Ser (D) Ala-Gly

Match the enzymes in Group I with their corresponding activity in Group II
Group                                 I Group II
P. Flippase                       1. Catalyzes the movement of any phospholipid across the lipid bilayer 
                                               down its concentration gradient
Q. Floppase                      2. Catalyzes the translocation of amino-phospholipids from the 
                                                extracellular to the inner leaflet
R. Lipase                           3. Catalyzes the translocation of membrane phospholipids from cytosolic to 
                                                the extracellular leaflet
S. Scramblase               4. Degradation of phospholipids from the lipid bilayer including the inner 
                                              and outer leaflets
(A) P-2, Q-3, R-4, S-1 (B) P-1, Q-2, R-3, S-4
(C) P-4, Q-1, R-2, S-3 (D) P-3, Q-2, R-4, S-1

Match the antibiotics in Group I with their mechanism of action in Group II
Group I                                            Group II
P. Tetracyclinetenone (C) Oligomycin (D) Antimycin A s             1. Inhibits bacterial protein synthesis by blocking peptidyl transfer
Q. Chloramphenicol       2. Inhibits bacterial protein synthesis by blocking the A-site on the ribosome
R. Cycloheximide           3. Misreads the genetic code and inhibits initiation of protein synthesis 
S. Streptomycin             4. Inhibits protein synthesis by blocking peptidyl transferase on 80S ribosome
(A) P-2, Q-1, R-3, S-4 (B) P-2, Q-1, R-4, S-3
(C) P-4, Q-3, R-1, S-2 (D) P-3, Q-4, R-2, S-1

Four proteins (P1, P2, P3 and P4) have 17, 10, 21 and 14 percent hydrophobic amino-acids 
respectively. The order of precipitation of these proteins using ammonium sulphate will be
(A) P3, P1, P4, P2 (B) P3, P1, P2, P4 (C) P2, P4, P3, P1 (D) P2, P4, P1, P3

Related  Gate 2014
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